Recording of DNA sequences to enable them to be expressed in yeasts, and the transformed yeasts obtained
US6787337B1 · kind B1 · utility
Assignee
Inventors
Key dates
| Filing date | Nov 15, 2000 |
| Grant date | Sep 7, 2004 |
| Priority date | — |
| Expiry date | Nov 15, 2020 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N15/81
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
The present invention relates to a recombinant non-yeast DNA, which encodes a protein of interest, wherein an unmodified DNA corresponding to the recombinant non-yeast DNA contains a region having a high content of codons that are poorly suited to yeasts, wherein a number of the codons that are poorly suited to yeasts are replaced in said region of the recombinant non-yeast DNA with synonymous codons coding for the same amino acid that are well-suited to yeasts, and wherein the number of replaced codons is sufficient to permit expression in yeasts. The present invention also relates to DNA sequences which originate from dicotyledonous or monocotyledonous plants, and in particular plants of the graminae family which are selected from among wheat, barley, oats, rice, maize, sorghum and cane sugar, as well as to vectors and transformed yeasts which contain the DNA sequences of the invention.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.