Compositions and methods for detecting raphidophytes
US6787648B2 · kind B2 · utility
Assignee
Inventors
Key dates
| Filing date | Feb 9, 2001 |
| Grant date | Sep 7, 2004 |
| Priority date | — |
| Expiry date | Jun 16, 2022 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12Q1/6895
- WIPO fieldOrganic fine chemistry
- WIPO sectorChemistry
Abstract
Effective management of finfish stocks to avoid or mitigate the threat of fish-killing phytoplankton is of increasing concern, particularly in temperate seas. Intensive spatial and temporal sampling is required to monitor and quantify potentially harmful species, so that prior warning can be received of an imminent bloom. The use of large-subunit rRNA (LSU rRNA)-targeted oligonucleotide probes based on the sandwich hybridization assay to detect the fragile species Heterosigma akashiwo (Hada) Hada and Fibrocapsa japonica Toriumi & Takano (Raphidophyceae) is disclosed. Species-specific sandwich hybridization assays were successfully developed for various Raphidophytes.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.