Adjustable sensitivity, genetic molecular interaction systems, including protein-protein interaction systems for detection and analysis
US6790607B1 · kind B1 · utility
3Cited by
2References
4Claims
0Family size
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Key dates
| Filing date | Oct 6, 2000 |
| Grant date | Sep 14, 2004 |
| Priority date | — |
| Expiry date | Oct 6, 2020 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N33/743
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
A method for detecting interactions between first and second interacting molecules at variable sensitivity. This variable sensitivity may be obtained by providing for the overexpression of either a bait hybrid protein containing a DNA binding domain (desensitization) or a prey hybrid protein containing the DNA activation domain for a reporter gene (enhanced sensitivity). The use of exogenous activators of one or the other according to the needs of a particular system is readily accomplished.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.