Method for chemical analysis of biological material

US6790669B1 · kind B1 · utility

Assignee

• THE DOW CHEMICAL COMPANY · US

Inventors

• Curtis D. Pfeiffer · Midland, US
• Nile N. Frawley · Midland, US
• Thomas L. Peters · Midland, US
• Philip J. Savickas · Franklin, US
• David R. Albers · Midland, US
• Steven J. Gluck · Lake Jackson, US
• Lawrence W. Nicholson · Midland, US
• Jose B. Esquivel H. · Midland, US

Key dates

• Filing date: Dec 14, 2001
• Grant date: Sep 14, 2004
• Expiry date: Mar 10, 2022

Classification

• Technology area (CPC Y): Emerging Cross-Sectional Technologies
• CPC primary: Y10T436/255
• WIPO field: Measurement
• WIPO sector: Instruments

Abstract

A chemical analysis method for determining chemically related differences between subject biological material such as genetically modified plant material and control biological material such as genetically unmodified plant material, which method includes at least the following six steps. The first step is to contact the subject biological material with a fluid extractant, such as a mixture of water, isopropanol and potassium hydroxide, to produce a fluid extract of the subject biological material. The second step is to contact the control biological material with the fluid extractant to produce a fluid extract of the control biological material. The third step is to chromatograph the fluid extract of the subject biological material, for example, gas or fluid chromatography, to produce a chromatogram of the fluid extract of the subject biological material. The fourth step is to chromatograph the fluid extract of the control biological material to produce a chromatogram of the fluid extract of the control biological material. The fifth step is to determine the differences between the chromatograms, for example, by using the method of U.S. Pat. No. 5,592,402, to identify at least one …