Synthesis of oligonucleotides

US6790946B2 · kind B2 · utility

Assignee

• Quiatech AB · SE

Inventors

• Marek Kwiatkowski · Uppsala, SE
• Ulf Landegren · Uppsala, SE
• Mats Nilsson · Uppsala, SE

Key dates

• Filing date: Sep 12, 2001
• Grant date: Sep 14, 2004
• Expiry date: Sep 12, 2021

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12Q1/6834
• WIPO field: Organic fine chemistry
• WIPO sector: Chemistry

Abstract

A method of preparing an immobilized oligonucleotide having a free 3′-end comprises the steps of: i) preparing an oligonucleotide attached in a first position to a solid support via its 3′-end and having a free 5′-end; ii) binding said oligonucleotide in a second position remote from the 3′-end to the solid support; and iii) selectively releasing the 3′-end of the oligonucleotide from the solid support to obta the oligonucleotide attached to the support in said second position in a reversed orientation with a free 3′-end.