Use of HPPD inhibitors as selection agents in plant transformation
US6791014B2 · kind B2 · utility
8Cited by
4References
17Claims
0Family size
Assignee
Inventors
Key dates
| Filing date | Aug 2, 2001 |
| Grant date | Sep 14, 2004 |
| Priority date | — |
| Expiry date | Aug 15, 2021 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N15/8209
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
The present invention provides a method for transforming plants cells by introducing a heterologous gene into competent plant cells with a gene for tolerance to HPPD inhibitors as a selection marker wherein a step for bleaching the competent plant cells is carried out prior to transforming the cells by introducing a suitable amount of HPPD inhibitor into the cell culture medium. The invention also provides methods for preparing transgenic plants comprising a heterologous gene.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.