Patent · US Expired

Broad range PCR amplification techniques

US6794133B1 · kind B1 · utility

14Cited by

4References

18Claims

0Family size

Assignee

The General Hospital Corporation · US

Inventors

Frederick M. Ausubel · Newton, US
Michael N. Mindrinos · Somerville, US
Eliana Drenkard · Cambridge, US

Key dates

Filing date	Jan 30, 2001
Grant date	Sep 21, 2004
Priority date	—
Expiry date	Jan 30, 2021

Classification

Technology area (CPC C)Chemistry; Metallurgy
CPC primaryC12Q1/6858
WIPO fieldBiotechnology
WIPO sectorChemistry

Abstract

Disclosed herein are methods and kits for determining whether a nucleic acid sequence includes a particular allele of a polymorphic sequence. These methods and kits focus on two sets of primers that are specific for an allele and are contacted with a nucleic acid sequence. One primer in each set is complementary to the polymorphic sequence at its 3′-terminal nucleotide and contains one or more mismatches in the five nucleotide adjacent to the 3′ terminus. The two sets of primers preferentially amplify methods are used to identify single nucleotide polymorphisms, for example, for genomic mapping purpose.

Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.