Modified DNA-polymerase from carboxydothermus hydrogenoformans and its use for coupled reverse transcription and polymerase chain reaction

US6794177B2 · kind B2 · utility

Assignee

• Roche Diagnostics GMBH · DE

Inventors

• Ursula Markau · Polling, DE
• Christine Ebenbichler · Antdorf, DE
• Gunthar Achhammer · Penzberg, DE
• Waltraud Ankenbauer · Penzberg, DE

Key dates

• Filing date: Feb 5, 2002
• Grant date: Sep 21, 2004
• Expiry date: Apr 14, 2022

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12N9/1252
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

A purified DNA polymerase exhibiting reverse transcriptase activity in the presence of magnesium ions and/or manganese ions having reduced or no 5′-3′-exonuclease activity and substantially no RNaseH activity and obtainable from Carboxydothermus hydrogenoformans.