Tryptase substrates and assay for tryptase activity using same

US6797461B2 · kind B2 · utility

Assignee

• Promega Corporation · US

Inventors

• Andrew L. Niles · Madison, US
• Mary Haak-Frendscho · Madison, US
• Jennifer Harris · San Diego, US
• Charles S. Craik · San Francisco, US

Key dates

• Filing date: Sep 19, 2001
• Grant date: Sep 28, 2004
• Expiry date: Sep 19, 2021

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C07K5/1024
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

The invention is directed to synthetic polypeptide substrates for tryptase enzymes and assays for tryptase activity that utilize the synthetic polypeptide substrates. The preferred synthetic polypeptide substrates are tetramers of the formula P4-P3-P2-P1, wherein the substrate is selected from the group consisting of P-R-N-K (SEQ. ID. NO: 2), P-K-N-K (SEQ. ID. NO: 3), P-R-N-R (SEQ. ID. NO: 4), P-K-N-R (SEQ. ID. NO: 5), P-A-N-K (SEQ. ID. NO: 6), and P-R-T-K (SEQ. ID. NO: 7).