Detection of nucleic acid sequence differences using coupled ligase detection and polymerase chain reactions

US6797470B2 · kind B2 · utility

Assignees

• Cornell Research Foundation, Inc. · US
• BOARD OF SUPERVISORS OF LOUISIANA STATE UNIVERSITY AND AGRICULTURAL AND MECHANICAL COLLEGE · US
• Regents of the University of Minnesota · US

Inventors

• Francis Barany · Baltimore, US
• Matthew Lubin · Rye Brook, US
• George Barany · Falcon Heights, US
• Robert P. Hammer · Baton Rouge, US

Key dates

• Filing date: Jul 30, 2001
• Grant date: Sep 28, 2004
• Expiry date: Jul 30, 2021

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12Q2600/16
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

The present invention relates to the detection of nucleic acid sequence differences using coupled ligase detection reaction and polymerase chain reaction. One aspect of the present invention involves use of a ligase detection reaction coupled to a polymerase chain reaction. Another aspect of the present invention relates to the use of a primary polymerase chain reaction coupled to a secondary polymerase chain reaction coupled to a ligase detection reaction. A third aspect of the present invention involves a primary polymerase chain reaction coupled to a secondary polymerase chain reaction. Such coupling of the ligase detection reaction and the polymerase chain reaction permits multiplex detection of nucleic acid sequence differences.