Imager for DNA sequencer
US6800438B2 · kind B2 · utility
Assignee
Inventors
Key dates
| Filing date | Dec 28, 2000 |
| Grant date | Oct 5, 2004 |
| Priority date | — |
| Expiry date | Dec 28, 2020 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N15/149
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
An improved method for the sequencing of DNA fragments is provided. The method includes using a known process for DNA fragment separation, such as capillary electrophoresis, and imaging the resultant gel plate with a full-width array scanner or a two-dimensional amorphous silicon image sensor array. The DNA sample is placed within a well of the separation apparatus, such as a capillary tube or plurality thereof. The separation apparatus is then placed in a buffer. An electric field is then applied, forming a bias between the ends along which the sample is separated. Once separated, the large area detector scans the entire gel plate and resultant image data is provided. By way of the improved method, the entire gel plate can be scanned at the same time and repeatedly, resulting in greater accuracy and a shorter time to sequence.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.