Methods and compositions for amplifying detectable signals in specific binding assays

US6806047B2 · kind B2 · utility

Assignee

• AFFYMETRIX, INC. · US

Inventors

• Martin J. Goldberg · Saratoga, US
• Govinda Rao S. Yelagalawadi · San Jose, US
• Eugene Yuji Tanimoto · Menlo Park, US
• Huu Tran · San Jose, US
• Helin Dong · Palo Alto, US
• David J. Lockhart · Mountain View, US
• Thomas B. Ryder · Escondido, US
• Janet Warrington · Los Altos, US
• Jody Beecher · Mountain View, US

Key dates

• Filing date: Feb 6, 2001
• Grant date: Oct 19, 2004
• Expiry date: Jul 14, 2021

Classification

• Technology area (CPC Y): Emerging Cross-Sectional Technologies
• CPC primary: Y10S436/807
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

Methods and compounds are provided for detecting target molecules in a sample using specific binding assays. In particular, methods are provided for detecting a nucleic acid target in a sample. In one embodiment, the method comprises hybridizing a nucleic acid target, comprising a target nucleic acid sequence, to a nucleic acid probe, comprising a probe nucleic acid sequence, wherein the target comprises a binding ligand. The hydridized target is contacted with a receptor comprising multiple sites capable of binding the binding ligand to complex the receptor to the binding ligand, and the receptor is contacted with an amplification reagent, comprising a plurality of the binding ligands, to complex the amplification reagent to the receptor. The presence of the complexed amplification reagent then is detected, for example, by detecting the presence of a detectable label, such as a fluorescent label, for example, on the receptor or the amplification reagent. Optionally, the amplification reagent, comprising a plurality of the binding ligands, is contacted with labeled receptor molecules thereby to complex a plurality of labeled receptor molecules to the amplification reagent, and the …