Fluorescent capture assay for kinase activity employing an anti-phosphotyrosine antibody as both the capture agent and detecting agent
US6806056B2 · kind B2 · utility
3Cited by
6References
9Claims
0Family size
Assignee
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Key dates
| Filing date | Apr 18, 2002 |
| Grant date | Oct 19, 2004 |
| Priority date | — |
| Expiry date | Jul 17, 2022 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N2333/91235
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
A method for determining the level of tyrosine kinase activity in a biological sample is disclosed. The method employs an anti-phosphotyrosine antibody as both the capture agent and the detecting agent. The detecting antibody is labeled with a fluorescent label, for instance, Cy5, Cy5.5 or Cy7 or a lanthanide ion, such as europium, as the signal generating entity. The method is particularly well suited to high throughput screening, for example, for compounds which modulate tyrosine kinase activity.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.