Optical sorting method
US6808882B2 · kind B2 · utility
143Cited by
5References
43Claims
0Family size
Assignee
Inventors
Key dates
| Filing date | Jun 29, 2001 |
| Grant date | Oct 26, 2004 |
| Priority date | — |
| Expiry date | Jun 29, 2021 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12Q1/6811
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
The invention describes a method for isolating one or more genetic elements encoding a gene product having a desired activity, comprising the steps of: (a) compartmentalising genetic elements into microcapsules; (b) expressing the genetic elements to produce their respective gene products within the microcapsules; (c) sorting the genetic elements which produce the gene product having the desired activity using a change in the optical properties of the genetic elements. The invention enables the in vitro evolution of nucleic acids and proteins by repeated mutagenesis and iterative applications of the method of the invention.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.