Methods for generating genetically altered antibody producing cell lines with improved antibody characteristics
US6808894B1 · kind B1 · utility
Assignee
Inventors
Key dates
| Filing date | Nov 7, 2000 |
| Grant date | Oct 26, 2004 |
| Priority date | — |
| Expiry date | Jul 16, 2021 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC07K2317/567
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
Dominant negative alleles of human mismatch repair genes can be used to generate hypermutable cells and organisms. By introducing these genes into cells and transgenic animals, new cell lines and animal varieties with novel and useful properties can be prepared more efficiently than by relying on the natural rate of mutation. These methods are useful for generating genetic diversity within immunoglobulin genes directed against an antigen of interest to produce altered antibodies with enhanced biochemical activity. Moreover, these methods are useful for generating antibody-producing cells with increased level of antibody production.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.