Retroviral vectors comprising a functional splice donor site and a functional splice acceptor site

US6808922B1 · kind B1 · utility

Assignee

• Oxford Biomedica, Inc. · GB

Inventors

• Christopher R. Bebbington · San Mateo, US
• Susan Kingsman · Felton, GB
• Mark Uden · Stevenage, GB
• Alan Kingsman · Shrewsbury, GB
• Kyriacos Mitrophanos · Shrewsbury, GB

Key dates

• Filing date: Jun 8, 2000
• Grant date: Oct 26, 2004
• Expiry date: Jun 8, 2020

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12N2840/44
• WIPO field: Pharmaceuticals
• WIPO sector: Chemistry

Abstract

A retroviral vector is described. The retroviral vector comprises a functional splice donor site and a functional splice acceptor site; wherein the functional splice donor site and the functional splice acceptor site flank a first nucleotide sequence of interest (“NOI”); wherein the functional splice donor site is upstream of the functional splice acceptor site; wherein the retroviral vector is derived from a retroviral pro-vector; wherein the retroviral pro-vector comprises a first nucleotide sequence (“NS”) capable of yielding the functional splice donor site and a second NS capable of yielding the functional splice acceptor site; wherein the first NS is downstream of the second NS; such that the retroviral vector is formed as a result of reverse transcription of the retroviral pro-vector.