Patent · US Expired

Method and system using acoustic ejection for preparing and analyzing a cellular sample surface

US6809315B2 · kind B2 · utility

28Cited by
17References
70Claims
0Family size

Assignee

Inventors

Key dates

Filing dateMar 1, 2002
Grant dateOct 26, 2004
Priority date
Expiry dateJul 4, 2022

Classification

  • Technology area (CPC Y)Emerging Cross-Sectional Technologies
  • CPC primaryY10T436/2575
  • WIPO fieldMeasurement
  • WIPO sectorInstruments

Abstract

The invention relates to a method and system for acoustically depositing a fluid on a surface of a cell sample. A reservoir containing a fluid is provided, and the cell sample surface is positioned in droplet-receiving relationship to the reservoir. Once the reservoir and the cell sample surface are appropriately positioned, focused acoustic energy is applied to eject a droplet of the fluid from the reservoir. As a result, the droplet is deposited on the sample surface at a designated site. Optionally, the fluid may be an analysis-enhancing fluid that contains a label moiety or a mass-spectrometry matrix material. When an analysis-enhancing fluid is used, the sample is typically subjected to conditions effective to allow the analysis-enhancing fluid to interact with the sample surface so as to render the sample surface suitable for analysis. Then, the sample may be analyzed at the designated site.

Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.