Method of monitoring cell motility and chemotaxis
US6811968B2 · kind B2 · utility
Assignee
Inventors
Key dates
| Filing date | Mar 15, 2002 |
| Grant date | Nov 2, 2004 |
| Priority date | — |
| Expiry date | Nov 6, 2022 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY10S435/81
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
The present invention discloses a method of monitoring chemotaxis or chemoinvasion comprising: providing a device for monitoring chemotaxis. The device includes a support member; a top member mounted to the support member by being placed in substantially fluid-tight, conformal contact with the support member. The support member and the top member are configured such that they together define a discrete assay chamber. The discrete assay chamber includes a first well region including at least one first well, the at least one first well being configured to receive a soluble test substance therein; a second well region including at least one second well, the at least one second well being configured to receive a sample comprising cells therein; and a channel region including at least one channel connecting the first well region and the second well region. Preferably, at least one second well is horizontally offset with respect to the first well region in a test orientation of the device. The method further comprises placing the soluble test substance in the at least one first well; forming a solution concentration gradient along a longitudinal axis of the chamber; placing a first sampl…
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.