Patent · US Expired

Process for the recombinant production of holo-citrate lyase

US6812019B1 · kind B1 · utility

0Cited by

0References

5Claims

0Family size

Assignee

Roche Diagnostics GMBH · DE

Inventors

Michael Bott · Jülich, DE
Peter Dimroth · Ludwigshafen, DE
Karin Schneider · Zürich, CH

Key dates

Filing date	Sep 28, 2000
Grant date	Nov 2, 2004
Priority date	—
Expiry date	Sep 28, 2020

Classification

Technology area (CPC G)Physics
CPC primaryG01N2333/988
WIPO fieldBiotechnology
WIPO sectorChemistry

Abstract

Process for the production of a protein with citrate lyase activity by expressing a suitable plasmid in a host organism and isolating the protein in an active form, wherein the plasmid contains the information from a gene cluster composed of at least six genes and an inducible promoter. Furthermore the invention concerns the use of the recombinant enzyme and a corresponding test kit for the determination of citric acid.

Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.