High efficiency mRNA isolation methods and compositions
US6812341B1 · kind B1 · utility
51Cited by
8References
36Claims
0Family size
Assignee
Inventor
Key dates
| Filing date | May 11, 2001 |
| Grant date | Nov 2, 2004 |
| Priority date | — |
| Expiry date | Jul 2, 2021 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N15/10
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
The present invention provides methods and compositions, including kits, for the isolation and purification of mRNA, particularly poly(A) RNA. It concerns the use of isostabilizing salts such as TMAC and TEAC to reduce rRNA carryover during the purification process, thus facilitating the isolation of poly(A) RNA.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.