Methods and probes for detection and/or quantification of nucleic acid sequences
US6815164B2 · kind B2 · utility
Assignee
Inventor
Key dates
| Filing date | Oct 9, 2001 |
| Grant date | Nov 9, 2004 |
| Priority date | — |
| Expiry date | Oct 17, 2021 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12Q1/6818
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
The present invention discloses nucleic acid detector probes for specific detection and/or quantification of target nucleic acid sequences and detection and/or quantification methods using these probes. In the absence of target nucleic acid sequence, a first oligonucleotide and a third oligonucleotide are bound to each other in a conformation which brings two member of an interacting moiety pair (labels) into close spatial proximity. Cooperative binding of the first oligonucleotide and a second oligonucleotide to a target nucleic acid sequence causes displacement of the third oligonucleotide from the first oligonucleotide probe resulting in separation of the two members of the interacting moiety pair (labels). The spatial separation of the moieties (labels) is detectable, and indicates the presence and/or amount of the target nucleic acid sequence. The method is useful for detection and/or quantification of a specific nucleic acid sequence as well as the detection of sequence alteration in the target nucleic acid sequence.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.