Amplification of DNA to produce single-stranded product of defined sequence and length
US6815167B2 · kind B2 · utility
39Cited by
6References
71Claims
0Family size
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Key dates
| Filing date | May 1, 2002 |
| Grant date | Nov 9, 2004 |
| Priority date | — |
| Expiry date | Sep 16, 2022 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12Q1/6853
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
The present disclosure relates to methods for generating single-stranded DNA molecules of defined sequence and length. Specifically, a region of template containing target sequence is amplified by PCR or RCA, exogenous sequence is introduced by primers or probes used in amplification, double-stranded amplification products are converted to single-stranded amplification products, and single-stranded amplification products are trimmed to produce short single-stranded DNA molecules of defined sequence and length.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.