Expression of biologically active polypeptide in duckweed
US6815184B2 · kind B2 · utility
40Cited by
9References
17Claims
0Family size
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Key dates
| Filing date | Jul 26, 2001 |
| Grant date | Nov 9, 2004 |
| Priority date | — |
| Expiry date | Nov 26, 2021 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N15/8257
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
Methods, nucleic acid sequences, and transformed duckweed plant or duckweed nodule cultures for the expression and the secretion of biologically active polypeptides from genetically engineered duckweed are provided. Expression of recombinant polypeptides in duckweed is improved by modifying the nucleotide sequence of the expression cassette encoding the polypeptide for improved expression in duckweed. Recovery of biologically active polypeptides from duckweed is improved by linking the biologically active polypeptide to a signal peptide that directs the secretion of the polypeptide into the culture medium.
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