Total protein detection methods and devices
US6815210B1 · kind B1 · utility
Assignee
Inventors
Key dates
| Filing date | Dec 11, 2003 |
| Grant date | Nov 9, 2004 |
| Priority date | — |
| Expiry date | Dec 11, 2023 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N33/543
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
An assay for the determination of protein in an aqueous test fluid which combines the test fluid with a buffer and a dye. The buffer is selected from citrulline, malonic acid, cyanoacetic acid, citraconic acid, methyl phosphonic acid, sarcosine, saccharin, or combinations thereof. The buffer is added in sufficient quantity to maintain the pH of the assay including the test fluid at a selected target pH range within a range of from about 2.0 to about 3.0. The dye has a pKa which enables it to operate as a protein indicator at the target pH range. The dye also has affinity for protein such that it will provide a detectable response in the presence of greater than about 15 mg/dL protein to thereby render the assay suitable for the detection of total protein in the test fluid. The buffer and dye may be absorbed in a test strip of absorbent material.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.