Coprecipitant and method for extracting nucleic acids
US6815541B1 · kind B1 · utility
Assignee
Inventors
Key dates
| Filing date | Apr 18, 1997 |
| Grant date | Nov 9, 2004 |
| Priority date | — |
| Expiry date | May 3, 2017 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N15/1003
- WIPO fieldOrganic fine chemistry
- WIPO sectorChemistry
Abstract
A coprecipitant and a nucleic-acid extraction method using the coprecipitant are provided. The coprecipitant has affinity to the nucleic acids, no competitive inhibition to the reverse transcription and no inhibition to the PCR reaction in case of extracting a very small amount of nucleic acids by alcoholic precipitation using isopropyl alcohol or ethanol. Further, the coprecipitant can precipitate with the nucleic acids as a visible white or blue precipitate, thereby to suppress technical errors and enhance the extraction efficiency.A coprecipitant which acts in a process of extracting the nucleic acids by centrifugal separation from biological materials and/or test samples in the same manner as nucleic acids and has ability for precipitating as a visible white or blue precipitate when being separating and concentrating it by alcohol.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.