Column device for isolation and labeling of nucleic acids
US6818398B2 · kind B2 · utility
Assignee
Inventors
Key dates
| Filing date | Dec 29, 2000 |
| Grant date | Nov 16, 2004 |
| Priority date | — |
| Expiry date | Jan 20, 2021 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12Q1/6816
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
A method for manipulating genetic material, the method comprising disrupting cells so as to liberate genetic material contained in the cells; contacting the genetic material to a silica column in a manner to cause the genetic material to become immobilized to the column; labeling the immobilized genetic material; and eluting the labeled material from the column. Also provided is a two-buffer process for manipulating genetic material, the process comprising: contacting cells containing the genetic material to a silica column; creating a first fraction of cell detritus and a second fraction containing the genetic material; confining the genetic material to the column; removing the cell detritus; contacting the genetic material with radicals so as to produce reactive aldehyde groups on the genetic material, and attaching chromophore to the genetic material.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.