DNA sequences coding for enzymes capable of facilitating the synthesis of linear &agr;-1,4 glucans in plants, fungi and microorganisms

US6818421B2 · kind B2 · utility

Assignee

• Bayer Bioscience GmbH · DE

Inventors

• Jens Kossmann · Golm, DE
• Volker Buttcher · Lauenförde, DE
• Thomas Welsh · Lawrence, US

Key dates

• Filing date: Apr 26, 2001
• Grant date: Nov 16, 2004
• Expiry date: Apr 26, 2021

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12N15/8246
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

The invention relates to DNA sequences coding for proteins having the enzymatic activity of an amylosucrase which allows the synthesis of linear &agr;-1,4 glucans from the substrate sucrose by bacteria, fungi and plants or in cell-free systems.The invention furthermore describes plasmids and bacteria containing these DNA sequences as well as processes for the production of plants and microorganisms capable of intracellularly or extracellularly expressing a polypeptide having amylosucrase activity.The invention furthermore relates to the production of pure fructose using proteins exhibiting the enzymatic activity of amylosucrase.