Method for increasing survival rate of cells in animal cell culture under hypoxia condition
US6818625B2 · kind B2 · utility
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6Claims
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Key dates
| Filing date | Jul 12, 2002 |
| Grant date | Nov 16, 2004 |
| Priority date | — |
| Expiry date | Jul 12, 2022 |
Classification
- Technology area (CPC A)Human Necessities
- CPC primaryA61P43/00
- WIPO fieldPharmaceuticals
- WIPO sectorChemistry
Abstract
The present invention relates to a method for increasing survival rate of cells in animal cell culture under hypoxia condition by adding antibiotics to the culture media. The method of present invention comprises a step of culturing animal cells in culture media containing antibacterial agent of quinolones, quinones, aminoglycosides or chloramphenicol at the concentration range of 0.1 to 1000 &mgr;g/ml. The invented method can be practically applied for high-density animal cell culture to produce recombinant proteins or cultured cells.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.