Immunoglobin-like domains with increased half lives

US6821505B2 · kind B2 · utility

Assignee

• The Board of Regents of the University of Texas System · US

Inventor

• Elizabeth Sally Ward · Cambridge, GB

Key dates

• Filing date: Aug 20, 2001
• Grant date: Nov 23, 2004
• Expiry date: Jan 19, 2022

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C07K2317/52
• WIPO field: Pharmaceuticals
• WIPO sector: Chemistry

Abstract

Disclosed are recombinant vectors encoding immunoglobulin-like domains and portions thereof, such as antibody Fc-hinge fragments, subfragments and mutant domains with extended biological half lives. Methods of producing large quantities of such domains, heterodimers, and fusion proteins following expression by host cells are also reported. Described are antibody Fc and Fc-hinge domains, which have the same in vivo stability as intact antibodies; and domains engineered to have increased in vivo half lives. These DNA constructs and protein domains will be useful as templates for in vitro mutagenesis and high resolution structural studies; for immunization and vaccination; and for the production of recombinant antibodies or chimeric proteins with increased stability and longevity for therapeutic and diagnostic uses.