Preparation of a recombinant protein in a prokaryotic host cell

US6821755B2 · kind B2 · utility

Assignee

• Boehringer Ingelheim International GmbH · DE

Inventors

• Rolf Werner · Ludwigshafen, DE
• Klaus Bergemann · Biberach, DE
• Friedrich Gotz · Tübingen, DE
• Andreas Peschel · Wolfratshausen, DE

Key dates

• Filing date: Jul 27, 2001
• Grant date: Nov 23, 2004
• Expiry date: Jul 27, 2021

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12P21/02
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

The invention relates to advantageous processes for preparing heterologous proteins in prokaryotic host cells by improved codon use and/or expression of tRNAs which code for codons occurring rarely in said host cell.