Rapid and simple process for isolation of circular nucleic acids

US6821757B2 · kind B2 · utility

Assignee

• QIAGEN GmbH · DE

Inventors

• Philippe Sauer · Pocking, DE
• Jie Kang · Bonn, DE

Key dates

• Filing date: Nov 18, 1999
• Grant date: Nov 23, 2004
• Expiry date: Nov 18, 2019

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C40B40/00
• WIPO field: Organic fine chemistry
• WIPO sector: Chemistry

Abstract

A method for separating and/or isolating circular nucleic acids from a mixture having different species of nucleic acids other than circular nucleic acids wherein the mixture is treated under alkaline conditions at a pH>8 with a solid matrix consisting essentially of a silica material in presence of at least one chaotropic substance.