Method for the production of biopolymers with modified properties

US6821758B1 · kind B1 · utility

Assignees

• Max-Planck-Gesellschaft zur Föderung der Wissenschaften e.V. · US
• DIREVO BIOTECH AG · DE

Inventors

• Andre Koltermann · Icking, DE
• Ulrich Kettling
• Manfred Eigen · Göttingen, DE

Key dates

• Filing date: Nov 9, 2000
• Grant date: Nov 23, 2004
• Expiry date: Nov 9, 2020

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12N15/1027
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

A method for the production of biopolymers with modified properties wherein at least one cycle comprising the following steps is completed:(a) providing a population of single-stranded polynucleotide molecules, wherein individual polynucleotide molecules comprise homologous and heterologous sequence segments and wherein individual ones of said single-stranded polynucleotide molecules can form double-stranded polynucleotide molecules with other ones of said single-stranded polynucleotide molecules within said population;(b) forming double-stranded polynucleotide molecules from the population of single-stranded polynucleotide molecules provided according to step (a) comprising double-stranded polynucleotide molecules with different heterologous sequence segments;(c) partially and exonucleolytically degrading the single-strands of the double-stranded polynucleotide molecules produced according to step (b); and(d) temple-directed single-stand synthesizing the degraded ends of the partially degraded double strand produced according to step (c),wherein steps (c) and (d) may be carried out sequentially or contemporaneously.