Methods for Agrobacterium-mediated transformation
US6822144B1 · kind B1 · utility
Assignee
Inventors
Key dates
| Filing date | Nov 3, 1997 |
| Grant date | Nov 23, 2004 |
| Priority date | — |
| Expiry date | Nov 3, 2017 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N15/8205
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
The present invention relates to methods for improving the transformation frequency of Agrobacterium-mediated transformation of maize embryos. A preferred method for transforming maize using Agrobacterium comprises the steps of: contacting at least one immature embryo from a maize plant with Agrobacterium capable of transferring at least one gene to said embryo; co-cultivating the embryos with Agrobacterium; culturing the embryos in medium comprising N6 salts, an antibiotic capable of inhibiting the growth of Agrobacterium, and a selective agent to select for embryos expressing the gene; and regenerating plants expressing the gene.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.