Ultra-sensitive detection systems using alterable peptide tags

US6824981B2 · kind B2 · utility

Assignee

• Agilix Corporation · US

Inventors

• Brian T. Chait · New York, US
• Darin Latimer · East Haven, US
• Paul M. Lizardi · San Antonio, US
• Eric Kershnar · New Haven, US
• Jon Morrow · Madison, US
• Matthew E. Roth · Branford, US
• Martin J. Mattessich · Woodbridge, US
• Kevin McConnell · Branford, US

Key dates

• Filing date: Aug 13, 2001
• Grant date: Nov 30, 2004
• Expiry date: Jan 24, 2022

Classification

• Technology area (CPC G): Physics
• CPC primary: G01N33/6803
• WIPO field: Measurement
• WIPO sector: Instruments

Abstract

Disclosed are compositions and methods for sensitive detection of one or multiple analytes. In general, the methods involve the use of special label components, referred to as reporter signals, that can be associated with, incorporated into, or otherwise linked to the analytes. In some embodiments, the reporter signals can be altered such that the altered forms of different reporter signals can be distinguished from each other. In some embodiments, sets of reporter signals can be used where two or more of the reporter signals in a set have one or more common properties that allow the reporter signals having the common property to be distinguished and/or separated from other molecules lacking the common property. In other embodiments, sets of reporter signal/analyte conjugates can be used where two or more of the reporter signal/analyte conjugates in a set have one or more common properties that allow the reporter signal/analyte conjugates having the common property to be distinguished and/or separated form other molecules lacking the common property. Reporter signals can also be in conjunction with analytes (such as in mixtures of reporter signals and analytes), where no significan…