Methods and reagents for inactivating ribonucleases
US6825340B2 · kind B2 · utility
21Cited by
4References
53Claims
0Family size
Assignee
Inventors
Key dates
| Filing date | Mar 23, 2001 |
| Grant date | Nov 30, 2004 |
| Priority date | — |
| Expiry date | Mar 23, 2021 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N9/99
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
The present invention is a general method for inactivating or inhibiting ribonucleases. Ribonucleases are treated with a reducing agent and heat. RNA samples contaminated with ribonuclease may be treated with this method to protect them from degradation. The RNA may then be used directly in a variety of enzymatic reactions and molecular biology techniques. This method may also be applied to a variety of molecular biology reagents which may be contaminated with ribonuclease to protect an RNA from being degraded when incubated with the reagent.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.