Conditionally immortalized cell lines derived from transgenic animals
US6825394B1 · kind B1 · utility
Inventors
Key dates
| Filing date | Oct 16, 1998 |
| Grant date | Nov 30, 2004 |
| Priority date | — |
| Expiry date | Oct 16, 2018 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N2517/02
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
The invention relates to mammalian cell lines and transgenic mammals. More particularly, it relates to a method for producing a rat cell line, a method for producing a transgenic rat, a transgenic rat, a rat cell line, cells and tissue obtained therefrom and uses therefore. The cell line derived from a transgenic mammal comprises: (i) a conditional oncogene, transforming gene or immortalising gene or a cell cycle affecting gene; and (ii) a cell type specific promoter. They include a neuronal cell line in which the cell type specific promoter is an NF-L gene promoter, and a mammary cell line in which the cell type specific promoter is a MMTV gene promoter. The conditional oncogene, transforming gene or immortalising gene is preferably a SV40tsA58 gene.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.