Method of identifying immunosuppressive agents
US6828091B2 · kind B2 · utility
Assignee
Inventors
Key dates
| Filing date | Aug 2, 2001 |
| Grant date | Dec 7, 2004 |
| Priority date | — |
| Expiry date | Oct 28, 2021 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N33/505
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
A method for identifying therapeutically effective immunosuppressive agents by screening such agents for those which induce apoptosis in activated T cells is disclosed. T cells were isolated then activated and treating with various test compounds. A caspase substrate is added to detect caspase activation and apoptosis in the cells. Compounds which stimulate caspase activation and apoptosis are also tested against resting T cells to determine those agents which are more effective in activated T cells compared to resting T cells. Compounds with this selectivity are effective in treating immunopathological disorders such as arthritis, graft rejection, graft versus host disease, inflammatory bowel syndrome and the like.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.