Protein fragment complementation assay (PCA) for the detection of protein-protein, protein-small molecule and protein nucleic acid interactions based on the E. coli TEM-1 &bgr;-Lactamase
US6828099B2 · kind B2 · utility
11Cited by
2References
31Claims
0Family size
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Key dates
| Filing date | May 31, 2001 |
| Grant date | Dec 7, 2004 |
| Priority date | — |
| Expiry date | Jun 20, 2022 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N2333/986
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
The present invention describes an assay method comprising: (A) generating (1) at least a first fragment of a reporter molecule linked to a first interacting domain and at least a second fragment of a reporter molecule linked to a second interacting domain, or (2) nucleic acid molecules that code for (A)(1) and subsequently allowing said nucleic acid molecules to produce their coded products; then, (B) allowing interaction of said domains; and (C) detecting reconstituted reporter molecule activity, where said reporter molecule can react with a penicillin- or a cephalosporin-class substrate.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.