Method of DNA sequencing
US6828100B1 · kind B1 · utility
657Cited by
6References
9Claims
0Family size
Assignee
Inventor
Key dates
| Filing date | Dec 4, 2001 |
| Grant date | Dec 7, 2004 |
| Priority date | — |
| Expiry date | Dec 4, 2021 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12Q1/6869
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
The present invention relates to a method of identifying a base at a target position in a sample nucleic acid sequence wherein a primer, which hybridizes to the sample nucleic acid immediately adjacent to the target position, is provided and the sample nucleic acid and primer are subjected to a polymerase reaction in the presence of a nucleotide whereby the nucleotide will only become incorporated if it is complementary to the base in the target position, and said incorporation is detected, characterized in that, a single-stranded nucleic acid binding protein is included in the polymerase reaction step.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.