Method of amplifying an RNA target sequence using an RNA polymerase functioning preferably on RNA template
US6828127B2 · kind B2 · utility
Assignee
Inventors
Key dates
| Filing date | Dec 8, 1999 |
| Grant date | Dec 7, 2004 |
| Priority date | — |
| Expiry date | Dec 8, 2019 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N9/127
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
Disclosed is a process for transcribing RNA using a nucleotide reagent as the promoter. Such a reagent enables any type of RNA to be transcribed without sequence specification and without protein cofactors, by means of an RNA polymerase that is known to be DNA-dependent such as the RNA polymerase of the phage T7, or by means of new, mutated RNA polymerase with the ability to synthesize a transcription product of a polynucleotide matrix with a higher yield when the matrix is RNA than when said matrix is DNA. This type of RNA polymerase can be obtained by effecting mutations on a coding gene for a wild-type RNA polymerase, and then by selecting the mutated RNA polymerase with said ability. The invention can be applied notably to the detection, synthesis or quantification of RNA.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.