Supports for the parallel identification and transcription profiling of polynucleic acids

US6830886B1 · kind B1 · utility

Assignee

• Memorec Medical Molecular Research Cologne Stoffel GmbH · DE

Inventors

• Andreas Bosio · Cologne, DE
• Wilhelm Stoffel · Cologne, DE
• Markus Stoffel · Herrliberg, CH

Key dates

• Filing date: Feb 1, 2001
• Grant date: Dec 14, 2004
• Expiry date: Feb 1, 2021

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C40B40/06
• WIPO field: Organic fine chemistry
• WIPO sector: Chemistry

Abstract

The invention relates to a support. Oligonucleotides or polynucleotides are covalently bound with the 5′- or 3′-termination on least one main surface of said support via bifunctional spacers and bifunctional linkers. The support is characterized in that the oligonucleotides or polynucleotides which are covalently bound with the 5′- or 3′-termination via bifunctional spacers and bifunctional linkers comprise 200 to 600 bp, and the oligonucleotides or polynucleotides can be obtained by using a method which comprises the following steps: Selecting homologous regions of mRNA of a target species and of at least one model species; selecting amplification primers which permit the amplification of 200 to 600, preferably 200 to 400 bp long nucleic acids from the homologous regions of both the mRNA of the target species and the mRNA of at least one model species, whereby the amplification primers optionally comprise a maximum of 1 mismatch per 6 nucleic acids of the amplification primer; immobilizing the nucleic acids on at the least one main surface of the support, said nucleic acids being obtained from the corresponding 200 to 600 bp long nucleic acids which are amp…