Glutaminase, its gene and a method of producing it
US6830905B2 · kind B2 · utility
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22Claims
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Key dates
| Filing date | Oct 2, 2002 |
| Grant date | Dec 14, 2004 |
| Priority date | — |
| Expiry date | Oct 2, 2022 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N9/80
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
Glutaminase is purified from an Aspergillus oryzae, its partial amino acid sequence is determined, a partial sequence of glutaminase gene is obtained by PCR based on the obtained information, and DNA fragments containing glutaminase gene from Aspergillus oryzae genomic library and cDNA library, and Aspergillus nidulans genomic library hybridization using the partial sequence as a probe.
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