Genetics units for inhibiting the function of RNA

US6830923B1 · kind B1 · utility

Assignee

• Boehringer Ingelheim International GmbH · DE

Inventors

• Hartmut Beug · Wien, AT
• Max L. Birnstiel · Wien, AT
• Matthew Cotten · Wien, AT
• Ernst-Werner Wagner · Langenzersdorf, AT
• Harald Kandolf · Wien, AT

Key dates

• Filing date: Sep 21, 1992
• Grant date: Dec 14, 2004
• Expiry date: Sep 21, 2012

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12N2310/121
• WIPO field: Pharmaceuticals
• WIPO sector: Chemistry

Abstract

The invention relates to a genetic unit, optionally present as a multiple-copy, for inhibiting RNA. The unit contains the transcription units necessary for transcription by polymerase III and a DNA coding for inhibiting RNA, which is arranged within the unit in such a way that the transcribed RNA is part of the polymerase III transcript. Using these units it is possible to achieve increased stability of the inhibiting RNA, which may occur in the form of ribozymes or antisense-RNAs, whilst maintaining an undiminished activity.The invention further relates to a process for introducing the genetic units into the cell, the use of these units and pharmaceutical preparations containing them.