Methods for isoelectric particle separation
US6833061B1 · kind B1 · utility
Assignee
Inventors
Key dates
| Filing date | May 24, 1999 |
| Grant date | Dec 21, 2004 |
| Priority date | — |
| Expiry date | May 24, 2019 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N2030/0035
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
In order to isoelectrically separate particles with a pH-dependent net charge, the particles are exposed in a guiding liquid to electric field forces. The pH value of the guiding liquid is set in such a way that at least one predetermined type of particle is separated from the remaining particles and migrates to a fixing collecting means under the effect of the electric field forces. The collecting means is for example a porous hollow fiber delimited by electrodes which generate the electric field forces and crossed by the guiding liquid together with the sample to be separated. The particles whose isoelectric point matches the pH value of the guiding liquid run unimpeded through the fibers, whereas the remaining particles are pressed against the inner wall of the fiber and are prevented from being carried away with the liquid flow.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.