Methods for detecting and sorting polynucleotides based on size
US6833242B2 · kind B2 · utility
Assignee
Inventors
Key dates
| Filing date | Apr 4, 2001 |
| Grant date | Dec 21, 2004 |
| Priority date | — |
| Expiry date | May 28, 2021 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N15/149
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
This invention relates in general to a method for molecular fingerprinting. The method can be used for forensic identification (e.g. DNA fingerprinting, especially by VNTR), bacterial typing, and human/animal pathogen diagnosis. More particularly, molecules such as polynucleotides (e.g. DNA) can be assessed or sorted by size in a microfabricated device that analyzes the polynucleotides according to restriction fragment length polymorphism. In a microfabricated device according to the invention, DNA fragments or other molecules can be rapidly and accurately typed using relatively small samples, by measuring for example the signal of an optically-detectable (e.g., fluorescent) reporter associated with the polynucleotide fragments.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.