Polynucleotide sequencing
US6833246B2 · kind B2 · utility
Assignee
Inventor
Key dates
| Filing date | Mar 29, 2002 |
| Grant date | Dec 21, 2004 |
| Priority date | — |
| Expiry date | Jun 8, 2022 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12Q1/6823
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
The invention relates to the sequencing of a target polynucleotide sequence, immobilized on a solid support, using the polymerase reaction to extend a suitable primer and characterizing the sequential addition of labelled bases. The present invention further relates to the presence of a polymerase enzyme that retains a 3′ to 5′ exonuclease function, which is induced to remove an incorporated labelled base after detection of incorporation. A corresponding non-labelled base may then be incorporated into the complementary strand to allow further sequence determinations to be made. Repeating the procedure allows the sequence of the complement to be identified, and thereby the target sequence.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.