Transgenic animals for producing specific isotypes of human antibodies via non-cognate switch regions
US6833268B1 · kind B1 · utility
Assignee
Inventors
Key dates
| Filing date | Jun 10, 1999 |
| Grant date | Dec 21, 2004 |
| Priority date | — |
| Expiry date | Jun 10, 2019 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N2830/00
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
The present invention provides fully human antibodies in a transgenic animal of a desired isotype in response to immunization with any virtually any desired antigen. The human immunoglobulin heavy chain transgene in the foregoing animals comprises a human constant region gene segment comprising exons encoding the desired heavy chain isotype, operably linked to switch segments from a constant region of a different heavy chain isotype, i.e., a non-cognate switch region. Said additional constant region segment comprises a switch region and human constant region coding segment, wherein the constant region coding segment is operably linked to a switch region that it is not normally associated with, i.e., a non-cognate switch region. In the transgenes of the invention, the non-cognate switch region may be a switch region from a different species than the constant region coding segment. The switch region and membrane exons of the invention may comprise a human gamma-2 constant region and the secreted constant region exons are from a human gamma-1 or a human gamma-4 constant region.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.