Assays for modulators of prolyl-4-hydroxylase
US6833489B1 · kind B1 · utility
0Cited by
0References
14Claims
0Family size
Assignee
Inventors
Key dates
| Filing date | Sep 15, 2000 |
| Grant date | Dec 21, 2004 |
| Priority date | — |
| Expiry date | Mar 12, 2021 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N2333/90245
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
A method for evaluating a test compound's ability to modulate prolyl 4-hydroxylase is disclosed. In one embodiment, the method comprises the steps of introducing a test compound into a test chimeric, P4H-gene modified, or a wild-type nematode, wherein the test chimeric nematode has a complemented prolyl-4-hydroxylase gene mutation, and observing the effect of the test compound on the prolyl 4-hydroxylase activity of the progeny of the test chimeric, P4H-gene modified, or the wild-type nematode, wherein a dpy or embryonic lethal phenotype indicates prolyl-4-hydroxylase inhibition.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.