Method of producing IgG
US6835379B2 · kind B2 · utility
Assignee
Inventors
Key dates
| Filing date | Dec 9, 2002 |
| Grant date | Dec 28, 2004 |
| Priority date | — |
| Expiry date | Jul 9, 2023 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC07K16/065
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
A method for producing IgG from plasma for medical applications, comprising at least: (i′) removal of albumin resulting in an IgG fraction, (ii′) purifying IgG from an IgG fraction, which is derived from the IgG fraction obtained in step (i′), by adsorbing IgG to a cation exchanger and collecting the adsorbed IgG fraction, and (iii′) virus inactivation in an IgG fraction derived from the IgG fraction collected in step (ii′). The method is characterized in; (I) concentrating the IgG fraction obtained in step (i′), (II) adjusting pH to 4±0.1 in the IgG fraction released from the cation exchanger in step (ii′), and preferably maintaining the pH below 6.0 during the remaining steps of the method; and (III) carrying out the virus inactivation (step iii′) by using chemicals at a temperature of 30° C.±2° C. for at least 4 hours. Anticomplementary activity is typically below 1 CH50/mg immunoglobulin.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.