Method of genetic modification of a wild type viral sequence
US6835538B1 · kind B1 · utility
Assignee
Inventors
Key dates
| Filing date | Apr 24, 2001 |
| Grant date | Dec 28, 2004 |
| Priority date | — |
| Expiry date | Apr 24, 2021 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N2770/00022
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
The present invention concerns a method of genetic modification of a TGB-3 wild type viral sequence for reducing or suppressing the possible deleterious effects of the agronomic properties of a transformed plant or plant cell by the TGB-3 viral sequence, comprising the following successive steps: submitting the sequence to point mutation(s) which allow the substitution of at least one amino-acid into a different amino-acid, selecting genetically modified TGB-3 wild type viral sequences having the point mutation(s) and which are not able to promote cell-to-cell movement of a mutant virus having a dysfunctional TGB-3 wild type viral sequence, when expressed in trans from a replicon, further selecting among the genetically modified TGB-3 viral sequences, the specifically genetically modified sequence which inhibits infection with a co-inoculated wild type virus when the mutant form was expressed from a replicon, and recovering the specifically genetically modified TGB-3 viral sequence.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.